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The Gravity Loading Countermeasure Skinsuit is an intravehicular activity suit for astronauts that has been developed to simulate some of the effects of Earth’s gravity and mitigate some of the physiological effects of microgravity, including spinal elongation, muscle atrophy, and sensorimotor changes. This wearable system is intended to supplement exercise during future missions to the Moon and Mars and to further attenuate microgravity induced physiological effects in future low-Earth orbit mission scenarios. The purpose of this study is to characterize the Skinsuit and its physiological effects on a short-duration low-Earth orbit mission.